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. 2014 Sep 29;9(9):e108181. doi: 10.1371/journal.pone.0108181

Figure 2. Proposed productive and non-productive interactions between TrCel7A and BC.

Figure 2

(A) The high affinity binding mode corresponds to the productive binding to cellulose chain ends on the hydrophobic face of BC microcrofibril through both domains, CBM and CD. (B) The medium affinity binding mode includes non-productive binding to the hydrophobic face, where enzyme is attached through CBM only. Latter can become productive binding upon disengagement of cellulose chain into the active site by endo-mode attack. (C) The low affinity binding mode may correspond to the active site mediated binding to cellulose chain ends on the hydrophilic face. The hydrophobic face of cellulose microfibril is shown in dark gray and the cellulose chain ends available for binding through CD are depicted as protruding lines. The A max and K d values for corresponding binding modes are listed in Table 1.