Table 1. Binding isotherm parameters for different binding modes of active site mediated and total binding of cellobiohydrolase TrCel7A to bacterial cellulose.

	High affinity binding modeb		Medium affinity binding modec		Low affinity binding moded
Parametera	Total bound	Active site bound	Total bound	Active site bound	Total bound	Active site bound
A max (nmol/g)	126±17	122±14	2440±260	790±50	-	-
K d (nM)	2.9±1.0	2.8±0.8	406±82	156±25	-	-
A max/K d (L/g)	43.1±14.7	43.3±12.7	6.0±1.2	5.1±0.8	0.21±0.01	0.22±0.01

^aParameter values were found by non-linear regression analysis of data in Figure 1. Error limits are the parameter errors from the non-linear regression route and are not primarily statistical in origin.

^bDataset was restricted with [TrCel7A]_Free up to 10 nM (Figure 1C) and Langmuiŕs one binding site model was used in analysis.

^cDataset was restricted with [TrCel7A]_Free up to 1.0 µM (Figure 1B) and Langmuiŕs two binding site model was used in analysis. Parameter values for the first, high affinity binding mode were fixed and the parameter values for the second, medium affinity binding mode were found by non-linear regression analysis.

^dFull dataset (n = 82) was analyzed according Langmuiŕs three binding site model (Figure 1A). Parameter values for the first two binding modes were fixed and the parameter values for the third, low affinity binding mode were found by non-linear regression analysis. Because of the low degree of saturation of this binding mode only the value of A _max/K _d can be found.