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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: Sci Signal. 2014 Sep 16;7(343):ra89. doi: 10.1126/scisignal.2005392

Fig. 9. Spatial and temporal dysregulation of pCbl in B cells expressing the Δ20–22 variant PLC-γ2-GFP.

Fig. 9

(A and B) Human B cells transfected with plasmids expressing either WT PLC-γ2-GFP or Δ20–22 PLC-γ2-GFP were labeled with DyLight 649-Fab anti-IgM, placed on anti-IgM bilayers for 5 or 15 min, fixed, stained with antibody specific for pCbl (Tyr700), and imaged by TIRF microscopy. (A) Representative TIRF images for the BCR (red) and pCbl (green), as well as merged images are shown. (B) Left: Means ± SEM of the MFI of pCbl. Right: Pearson’s colocalization coefficients for the BCR and pCbl. Each data point represents the values from a cell analyzed in one of two independent experiments. Statistical analysis was performed by ANOVA.