(A) Heat map of differentially regulated genes pertaining to tumor immunity in early and late treated tumors. (B-D) Percent Teff (B), Treg (C) and Teff/Treg ratio (D) in early and late treated tumors. (E) Relative Ctla4 expression in late treated tumors (n=6). (F-G) CD8+ cytotoxic T cell/Treg ratio (F) and CD3/CD11b ratio (G) in early and late treated tumors. (H, I) Percent Teff (H) and Treg (I) in normal pancreas and in early and late treated tumors. (J-L) Percent CD11b+Ly6G+ (J), CD11b+Gr1+F4/80- (K) and CD11b+Gr1-F4/80+ (L) cells in late treated tumors. Data is represented as mean +/- SEM. Significance was determine by t-test except for multiple groups comparison, in which significance was determined by One-way ANOVA with Turkey post-hoc analysis, * p<0.05, ** p<0.01, ** p< 0.001, ****p < 0.0001. ns: not significant. See also Figure S5.