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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Am J Med Genet A. 2010 Apr;0(4):807–814. doi: 10.1002/ajmg.a.33342

Figure 4.

Figure 4

Functional characterization of the MEK2 p.P128Q mutant. Human embryonic kidney 293T cells were transiently transfected with the MEK2 p.P128Q mutant plasmid and appropriate controls. ERK phosphorylation was assayed by Western blotting using phosphospecific antibodies. The p.P128Q MEK2 mutant protein had increased ERK phosphorylation compared to the level induced by empty vector, wild-type MEK2 and the kinase dead control. However, the p.P128Q MEK2 mutant protein was less active less than the CFC MEK2 p.F57C mutant which is known to have increased activity over wild-type. This indicates that p.P128Q MEK2 is a weak hypermorph. Myc-tagged MEK2 served as a marker for transfection efficiency and total ERK served as a loading control.