Skip to main content
. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
HEPES-KOH, pH 7.5 50 mM 1 M 50 ml
KCl 300 mM 1 M 300 ml
NP40 substitute 0.05% (v/v) 100% 0.5 ml
H2O 649 ml
DTT (add fresh) 0.5 mM 1 M 0.5 ml
Complete EDTA-free protease inhibitor cocktail (add fresh) 1 tablet/50 ml