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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
Tris–HCl, pH 7.9 50 mM 1 M 50 ml
NaCl 100 mM 3 M 33.3 ml
MgCl2·6H2O 10 mM 1 M 10 ml
H2O 906.2 ml
DTT (add fresh) 1 mM 1 M 0.5 ml