Component | Final concentration | Stock | Amount |
---|---|---|---|
Tris–HCl, pH 7.5 | 50 mM | 1 M | 50 ml |
NaCl | 50 mM | 3 M | 16.7 ml |
MgCl2·6H2O | 10 mM | 1 M | 10 ml |
H2O | 918.3 ml | ||
DTT (add fresh) | 5 mM | 1 M | 5 ml |
Component | Final concentration | Stock | Amount |
---|---|---|---|
Tris–HCl, pH 7.5 | 50 mM | 1 M | 50 ml |
NaCl | 50 mM | 3 M | 16.7 ml |
MgCl2·6H2O | 10 mM | 1 M | 10 ml |
H2O | 918.3 ml | ||
DTT (add fresh) | 5 mM | 1 M | 5 ml |