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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
Proteinase K 20 mg ml−1 200 mg
Tris–HCl, pH 8 50 mM 1 M 0.5 ml
CaCl2·2H2O 30 mM 1 M 30 μl
Glycerol 50% 100% 5 ml
H2O to 10 ml