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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Consumables 1.5-ml polypropylene tubes
1.5-ml siliconized tubes (BIO PLAS Inc., 4165SL)
15- and 50-ml conical tubes (e.g., Falcon) as well as tubes withstanding high-speed centrifugation (e.g., Sarsted, 13-ml centrifuge tube, 55.518)
15-cm culture dishes
10-cm tissue culture dishes
5-μm Supor membrane syringe filter (Pall Acrodisc)
Cell scraper (Corning)
D-Tube Dialyzer Midi, MWCO 3.5 kDa (EMD Biosciences, 71506-3)
NuPAGE Novex 4–12% BT Midi 1.0 gel (Invitrogen)
pH paper (covering the range between pH 6.5 and 10)
Plastic wrap
Scalpels or razor blades
Strips of 0.2-ml tubes (Thermo Scientific, AB-0264)
Syringes (10 ml)