Consumables |
1.5-ml polypropylene tubes |
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1.5-ml siliconized tubes (BIO PLAS Inc., 4165SL) |
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15- and 50-ml conical tubes (e.g., Falcon) as well as tubes withstanding high-speed centrifugation (e.g., Sarsted, 13-ml centrifuge tube, 55.518) |
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15-cm culture dishes |
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10-cm tissue culture dishes |
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5-μm Supor membrane syringe filter (Pall Acrodisc) |
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Cell scraper (Corning) |
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D-Tube Dialyzer Midi, MWCO 3.5 kDa (EMD Biosciences, 71506-3) |
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NuPAGE Novex 4–12% BT Midi 1.0 gel (Invitrogen) |
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pH paper (covering the range between pH 6.5 and 10) |
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Plastic wrap |
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Scalpels or razor blades |
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Strips of 0.2-ml tubes (Thermo Scientific, AB-0264) |
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Syringes (10 ml) |