PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a Step-By-Step Protocol to the Transcriptome-Wide Identification of Binding Sites of RNA-Binding Proteins

. Author manuscript; available in PMC: 2014 Oct 2.

Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6

Combine 25-ml acidic phenol, 24-ml chloroform and 1-ml isoamyl alcohol (overlay with 0.1-M citrate buffer, pH 4.3 ± 0.2 which you can take from the acidic phenol bottle)