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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
Tris–HCl, pH 7.6 0.5 M 1 M 5 ml
MgCl2·6H2O 0.1 M 1 M 1 ml
2-Mercaptoethanol 0.1 M 14.3 M 0.07 ml
Acetylated BSA 1 mg ml−1 20 mg ml−1 0.5 ml
H2O 3.43 ml