| Component | Final concentration | Stock | Amount |
|---|---|---|---|
| Tris base | 445 mM | 53.9 g | |
| Boric acid | 445 mM | 27.5 g | |
| EDTA–NaOH, pH 8.0 | 10 mM | 0.5 M | 20 ml |
| H2O to 1 l |
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