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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
Tris–HCl, pH 8.0 100 mM 1 M 1 ml
KCl 500 mM 1 M 5 ml
2-Mercaptoethanol 10 mM 14.3 M 7 μl
Triton X-100 1% (v/v) 100% 0.1 ml
MgCl2·6H2O 20 mM 1 M 2 ml
H2O 1.9 ml