Component | Final concentration | Stock | Amount |
---|---|---|---|
Tris–HCl, pH 8.0 | 100 mM | 1 M | 1 ml |
KCl | 500 mM | 1 M | 5 ml |
2-Mercaptoethanol | 10 mM | 14.3 M | 7 μl |
Triton X-100 | 1% (v/v) | 100% | 0.1 ml |
MgCl2·6H2O | 20 mM | 1 M | 2 ml |
H2O | 1.9 ml |
Component | Final concentration | Stock | Amount |
---|---|---|---|
Tris–HCl, pH 8.0 | 100 mM | 1 M | 1 ml |
KCl | 500 mM | 1 M | 5 ml |
2-Mercaptoethanol | 10 mM | 14.3 M | 7 μl |
Triton X-100 | 1% (v/v) | 100% | 0.1 ml |
MgCl2·6H2O | 20 mM | 1 M | 2 ml |
H2O | 1.9 ml |