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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2014;539:113–161. doi: 10.1016/B978-0-12-420120-0.00008-6
Component Final concentration Stock Amount
HEPES-KOH, pH 7.5 50 mM 1 M 50 ml
KCl 150 mM 1 M 150 ml
EDTA–NaOH, pH 8.0 2 mM 0.5 M 4 ml
NaF 1 mM 0.5 M 2 ml
NP40 substitute 0.5% (v/v) 100% 5 ml
H2O 788.5 ml
DTT (add fresh) 0.5 mM 1 M 0.5 ml
Complete EDTA-free protease inhibitor cocktail (add fresh) 1 tablet/50 ml