Component | Final concentration | Stock | Amount |
---|---|---|---|
HEPES-KOH, pH 7.5 | 50 mM | 1 M | 50 ml |
KCl | 150 mM | 1 M | 150 ml |
EDTA–NaOH, pH 8.0 | 2 mM | 0.5 M | 4 ml |
NaF | 1 mM | 0.5 M | 2 ml |
NP40 substitute | 0.5% (v/v) | 100% | 5 ml |
H2O | 788.5 ml | ||
DTT (add fresh) | 0.5 mM | 1 M | 0.5 ml |
Complete EDTA-free protease inhibitor cocktail (add fresh) | 1 tablet/50 ml |