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. Author manuscript; available in PMC: 2015 Sep 5.
Published in final edited form as: Chembiochem. 2014 Jul 8;15(13):1887–1890. doi: 10.1002/cbic.201402224

Table 1.

Thermal melting dataa for PNA oligomers with complementary DNA and alone.

PNA Sequenceb Tm[°C]
DNAc 		Tm[°C]
Quadruplexd
4 H2N-Glu-G-G-G-C-G-G-A-G-G-Glu-Lys 82.4 54.0
8 H2N-Glu-G-X-G-C-G-X-A-G-G-Glu-Lys 77.0 -
9 H2N-Glu-G-X-G-C-G-X-A-G-G-Glu-Lys 88.0 -
a

Solutions of PNA/DNA (1:1) were prepared in pH 7.0 PBS buffer containing 10 mM sodium phosphate, 0.1 mM EDTA, and 150 mM NaCl.

b

PNA oligomers are written from N terminus to C terminus. Lys = lysine, Glu = glutamic acid.

c

Complementary antiparallel DNA 5′-CCT CCG CCC-3′. Strand concentration is 5 microM for both PNA and DNA. Tm is estimated from the inflection point in the melting curve monitored at 260 nm. Estimated error is +/- 0.5 °C.

d

PNA concentration is 10 microM. Tm is estimated from the inflection point in the melting curve (reverse sigmoidal) monitored at 295 nm. Estimated error is +/- 0.5 °C.