Figure 4. Specific monoclonal antibodies inhibit gp120 binding at protein or integrin level.

CD4⁺ cells (left) and CD8⁺ cells (right) were incubated with biotinylated gp120 from clade A either without antibody pretreatment, red line (control) or after pretreatment with 2 µg of anti-integrin α4 mAb, HP2/1, (blue line), anti-V2 mAb 697D (orange line) or anti-V2 mAb 2158 (aqua). Binding was detected with NeutrAvadin PE and analyzed by FACS. The binding of clade A gp120 (red line) is inhibited by the addition of 2 µg anti-α4 mAb (blue line), anti-V2 mAb 697D (orange line) and anti-V2 mAb 2158 (aqua line). Green histogram is NeutrAvidin control, LFI = Log Fluorescence Intensity.