Figure 1. In situ hybridisation-based detection of telomeres and the H. chilense chromosome 3H^ch subtelomeric regions in addition line root tip metaphase spreads.

(a) The line carrying the version of chromosome 3H^ch in which a deletion on the long arm has removed neither the long arm telomere nor the subtelomeric region, (b) the line carrying the version of chromosome 3H^ch in which a deletion on the short arm has removed the subtelomeric region. The 3H^ch chromosomes (indicated by a white arrow) can be distinguished by the presence of signal following hybridisation with HvT01, as confirmed by probing with labelled H. chilense genomic DNA. Bar: 10 µm.