Table 2.
PCR primer sequences of the target genes
| Target genes | Reference/Genbank accession | PCR products (bp) | Primer sequences |
|---|---|---|---|
| GAPDH | HM043737.1 | 180 | F: 5’-GGGTCATCATCTCTGCACCT -3’ |
| R: 5’-GGTCATAAGTCCCTCCACGA -3’ | |||
| Occludin | BC133617.1 | 200 | F: 5’-GTTCGACCAATGCTCTCTCAG -3’ |
| R: 5’-CAGCTCCCATTAAGGTTCCA -3’ | |||
| Claudin-1 | HM117762.1 | 216 | F: 5’-CACCCTTGGCATGAAGTGTA-3’ |
| R: 5’-AGCCAATGAAGAGAGCCTGA -3’ | |||
| Claudin-4 | HM117763.1 | 238 | F: 5’-AAGGTGTACGACTCGCTGCT-3’ |
| R: 5’-GACGTTGTTAGCCGTCCAG-3’ | |||
| β-defensin | HM593790.1 | 165 | F: 5’- CTGCTGGGTCAGGATTTAC -3’ |
| R: 5’- GCGTCTTCGCCTTCTGTT -3’ | |||
| Bcl-2 | AY423861.1 | 208 | F: 5’- TCGCCCAAGTCAAACATTA-3’ |
| R: 5’- CACAGGTGAAACTGCCAAGAT-3’ | |||
| Bax | AF163774.1 | 178 | F: 5’-TGCTCACTGCCTCACTCAC-3’ |
| R: 5’-CCAAGACCACTCCTCCCTA-3’ |