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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2012;502:43–66. doi: 10.1016/B978-0-12-416039-2.00003-3

Figure 1. Phage antibodies are endocytosed into ErbB2 expressing cells.

Figure 1

Top panel shows different phage antibody constructs studied. Bottom panel shows immunofluorescent microscopy, staining for phage major coat protein pVIII. Panels A–D phage are displayed in a phagemid vector where there is a single scFv/phage. Panel E phage are displayed in a phage vector with 3–5 copies of scFv/phage. A. Control phage antibody (binds BoNT). B. C6.5 anti-ErbB2 scFv. C. Higher affinity ML3-9 anti-ErbB2 scFv. D. Dimeric C6.5 diabody. E. C6.5 scFv displayed multivalently in true phage vector.