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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2012;502:43–66. doi: 10.1016/B978-0-12-416039-2.00003-3

Figure 3. Cartoon of selections to direct internalizing antibodies to a specific target antigen.

Figure 3

A. The phage library is selected for two rounds for internalization into a mammalian cell. B. The polyclonal phage from the 2nd round of selection is applied to yeast displaying the target antigen. C. Unbound phage are removed by washing. D. Bound phage are eluted by incubating yeast with low pH. E. Eluted phage are used to infect E. coli to amplify phage for the next round of selection. F. Selections are repeated for 2–3 rounds.