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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Methods Enzymol. 2012;502:43–66. doi: 10.1016/B978-0-12-416039-2.00003-3

Figure 4. Display of antigen domains on the surface of yeast.

Figure 4

(A) The extracellular domain (ECD) of receptor EphA2 was displayed on the yeast surface and recognized by anti-EphA2 antibody and recombinant mouse Ephrin A1 (R&D) as determined by flow cytometry analysis. (B) The link domain of CD44 (domain 1, or D1) was displayed on the yeast surface and recognized by anti-CD44 rabbit monoclonal antibody as determined by flow cytometry analysis. Both anti-EphA2 and anti-CD44 antibodies did not recognize an irrelevant protein displayed on the yeast surface. The unstained yeast in quadrant 1 represent the parental yeasts that did not induce.