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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Fungal Genet Biol. 2010 Jan 4;47(4):318–331. doi: 10.1016/j.fgb.2009.12.011

Fig. 4. Functional and immunological localization of sPlaA and sPlaB in overexpressing strains.

Fig. 4

(A) Vector-transformed control strain NGA142-1, OESA-1, and OESB-1 were cultured in DPY medium for 3 days, followed by culture medium recovery and lysate preparation. PLA2 activity in each fraction (culture media: open bars; lysates: closed bars) was measured at both pH 5.5 and pH 7.4 in reaction mixtures supplemented with 10 mM Ca2+. (B) Immunoblot analysis of culture media and lysates prepared from OESA-1 and OESB-1 using the anti-sPlaA and the anti-sPlaB antibodies as indicated.