Table 2.

Global phospholipid profiles of the sPLA₂-expressing and control strains

	NGA 142-1a	OESA-1a	OESB-1a
PHOSPHOLIPIDS
Polar head groupsb
Choline	41.91	39.50	41.32
Ethanolamine	35.50	34.40	31.35
Inositol	12.71	14.20	14.60
Serine	7.74	9.72	10.33
Phosphatidic Acid	2.09	2.27	2.36
Di-fatty acyl groups (N. of carbons)ce
32	0.28	0.40	0.38
33	1.19	0.93	0.97
34	38.75	41.41	42.02
35	2.24	1.76	1.57
36	52.20	47.90	48.68
37	0.32	0.25	0.23
38	1.24	1.02	0.86
40	1.35	1.07	1.30
41	1.93	4.29	3.71
42	0.36	0.83	0.21
Degree of unsaturation (N. of double bonds)de
0	0.35	0.50	0.38
1	6.51	8.29	8.67
2	37.56	37.76	37.50
3	13.09	13.73	14.60
4	34.03	28.10	28.20
5	7.54	10.49	9.78
6	0.52	0.94	0.70
7	0.22	0.15	0.14
LYSOPHOSPHOLIPIDS
Polar head groupsb
Choline	64.61(0.52)f	58.50 (0.46)	61.09 (0.57)
Ethanolamine	27.89 (0.22)	26.50 (0.21)	25.34 (0.24)
Lysophosphatidic acid	7.50 (0.06)	15.00 (0.12)	13.57 (0.13)
Fatty acyl groups (N. of carbons)c, e
14	0.05	0.34	0.31
15	0.56	0.57	0.57
16	28.24	34.29	30.75
17	1.40	1.32	0.85
18	69.17	62.71	67.14
20	0.58	0.78	0.39
Degree of unsaturation (N. of double bonds)d, e
0	31.06	37.10	34.17
1	9.82	11.47	11.80
2	54.95	47.09	50.38
3	4.12	4.29	3.62

^aThe control (NGA 142-1), sPlaA- (OESA-1) and sPlaB- (OESB-1) expressing strains were subjected to PL, and LysoPL analysis.

^bRelative polar head group abundance (mol %) in the total PL or LysoPL pool.

^cRelative abundance (mol %) of the indicated di-fatty acyl or mono-fatty acyl groups in the total PL or LysoPL pool as indicated.

^dRelative abundance (mol %) of di-fatty acyl or mono-fatty acyl groups with different degrees of unsaturation in the total PL or LysoPL pool as indicated.

^eOnly fatty acyl species with a relative abundance >0.1% are reported.

^fIndicated in brackets are LysoPL polar head group abundance values expressed as nmol/mg dry weight (see ‘Experimental procedures’ for details).