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. 2013 Sep 11;14(3):357–368. doi: 10.1016/j.chom.2013.08.004

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© 2013 The Authors. Published by Elsevier Inc.

This work is licensed under a Creative Commons Attribution 3.0 Unported License.

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An In Vivo Assay for the Analysis of Kupffer Cell Behavior

(A) Intravital analysis of KC (green) in liver sinusoids (red) in naive (LysM-Cre × mT/mG)_F1 mice. Arrows indicate moving monocytes and/or neutrophils. Arrow heads show stationary KCs. See also Movie S1.

(B–E) Measurement of the parameters of cell volume (B), surface area (C), shape factor (D), and velocity (E) for single KCs in 3 dimensions over time. Data represent 42 KCs from 2 mice.

(F–I) Shown are the average volume (F), surface area (G), shape factor (H), and velocity (I) over time for KC, LSEC, and monocytes and/or neutrophils. Data are represented as mean ± SEM. Data represent 35 KCs, 8 LSECs, and 19 monocytes and/or neutrophils from 2 mice.