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. 2014 Sep 29;9(9):e108372. doi: 10.1371/journal.pone.0108372

Figure 9. EC does not cause aggregation of salivary mucins.

Figure 9

Rate-zonal centrifugation of human whole saliva with 1 mM EC (grey triangles) or PBS (black circles), performed in 10–50% (w/v) sucrose gradients in PBS pH 7.4. (A) MUC5B was detected by agarose gel electrophoresis of rate-zonal fractions and western blotting with the MAN-5BIII antiserum (inset; saliva (top panel) and saliva+EC (bottom panel)). (B) MUC7 was detected by agarose gel electrophoresis of rate-zonal fractions and western blotting with the EUMUC7a antiserum (inset; saliva (top panel) and saliva+EC (bottom panel)). Bands were quantified using the Odyssey Imaging system.