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. 2014 Sep 29;9(9):e108431. doi: 10.1371/journal.pone.0108431

Table 1. Probes with the largest over-expression or under-expression values achieved at the final stress point. Data represents the ratio of change in expression at the final stress level of 50% relative to that at 90 leaf %RWC taken from the ANOVA group dataset stress+line (File S4). Transcript profile changes, as a function of leaf %RWC, were similar across the stress transient for the three wheat lines and probes were annotated to MapMan pathways and bins.

MapMan MapMan Affymetrix MapMan Expression Change
Bin# Pathway Probe Annotation 50∶90
1 Photosynthesis ta.3252.1.s1_at PS2 polypeptide subunits 1.34
2 Sugar metabolism ta.11114.1.a1_at ATBETAFRUCT4/VAC-INV (VACUOLAR INVERTASE) 0.93
3 Minor sugars ta.20649.2.s1_a_at ATTPS6 (Arabidopsis thaliana trehalose phosphatase/synthase 6) 0.83
4 Glycolysis taaffx.80151.1.s1_at PFK2 phosphofructokinase family protein 1.08
5 Fermentation taaffx.734.1.s1_x_at ALDH7B4 (ALDEHYDE DEHYDROGENASE 7B4) 0.93
6 Gluconeogenesis ta.23970.1.a1_at MASY malate synthase 1.59
7 OPP ta.9372.1.s1_at transaldolase 0.57
8 Tricarboxylic acid cycle ta.2022.2.s1_at aconitate hydratase, cytoplasmic, putative 0.35
9 Mitochondrial electron transport taaffx.121853.1.s1_at AOX1C (alternative oxidase 1C) 0.70
10 Cell wall taaffx.7177.1.s1_s_at glycosyl hydrolase family 3 protein 1.91
11 Lipid metabolism ta.9528.1.a1_at oxidoreductase, acting on the CH-CH group of donors 0.91
12 N metabolism ta.1870.1.s1_a_at GDH2 (GLUTAMATE DEHYDROGENASE 2) 0.78
13 Amino acid metabolism taaffx.95414.1.s1_at ATBCAT-3/BCAT3 (BRANCHED-CHAIN AMINOTRANSFERASE 3) 1.50
14 S assimilation ta.11132.1.s1_at adenylylsulfate kinase 0.14
15 Metal handling ta.17991.1.s1_x_at binding, chelation and storage 0.49
16 Secondary metabolism ta.11147.1.a1_at FAH1 (FERULATE-5-HYDROXYLASE 1) 1.11
17 Hormones taaffx.12175.1.s1_at ATGA2OX1 (GIBBERELLIN 2-OXIDASE 1) 1.60
18 Cofactors and vitamins ta.13745.1.s1_a_at LIP1 (LIPOIC ACID SYNTHASE 1 0.10
19 Tetrapyrrole biosynthesis ta.11708.1.s1_at HEMD; uroporphyrinogen-III synthase 0.08
20 Abiotic stress ta.1316.2.s1_x_at universal stress proteins (USP)family protein 1.26
20 Stress (biotic and abiotic) ta.13785.1.s1_at (CHIB1) acidic endochitinase 1.66
21 Redox ta.5610.1.s1_at AHB1 (ARABIDOPSIS HEMOGLOBIN 1) 1.04
22 Polyamine synthesis ta.3005.1.s1_a_at ATAIH/EMB1873 (AGMATINE IMINOHYDROLASE) 0.13
23 Nucleotide metabolism taaffx.97704.1.a1_at EMB2742 (EMBRYO DEFECTIVE 2742) 0.73
24 Degradation of xenobiotics ta.8571.1.s1_a_at lactoylglutathione lyase family protein/glyoxalase I family protein 1.83
25 C1 metabolism ta.20549.1.s1_x_at FDH (FORMATE DEHYDROGENASE 0.32
26 Misc enzyme families ta.11025.1.a1_at FAD-binding domain-containing protein 1.41
27 RNA processing ta.26049.1.s1_a_at MYB4 (myb domain protein 4); transcription factor 1.39
28 DNA synthesis taaffx.128682.1.s1_at ENDO4 (ENDONUCLEASE 4) 1.45
29 Protein modification ta.3507.1.s1_at SCPL16 (serine carboxypeptidase-like 16) 1.64
30 Signalling ta.4334.1.s1_at CPK6 (CALCIUM-DEPENDENT PROTEIN KINASE 6) 1.55
31 Cell cycle, division, vesicle transport and organisation ta.214.1.s1_at TUB6 (BETA-6 TUBULIN) 1.37
33 Development ta.13396.1.s1_at late embryogenesis abundant 1.87
34 Transport ta.9295.1.a1_at PGP21 (P-GLYCOPROTEIN 21); ATPase, coupled to transmembrane 1.42
1 Photosynthesis ta.22984.2.s1_x_at LHB1B2 (Photosystem II light harvesting complex gene 1.5); chlorophyll binding −1.28
2 Sugar metabolism ta.1197.1.s1_x_at ATPHS2/PHS2 (ALPHA-GLUCAN PHOSPHORYLASE 2) −1.04
3 Minor sugars ta.12952.2.s1_at pfkB-type carbohydrate kinase family protein −0.65
4 Glycolysis ta.9096.2.s1_x_at ATPPC1 (PHOSPHOENOLPYRUVATE CARBOXYLASE 1) −0.25
5 Fermentation ta.28355.2.s1_x_at ADH2 (ALCOHOL DEHYDROGENASE 2) −0.52
6 Gluconeogenesis ta.37.1.s1_at PMDH2 (PEROXISOMAL NAD-MALATE DEHYDROGENASE 2) −0.36
7 OPP ta.29329.1.s1_at phosphogluconate dehydrogenase −0.56
8 Tricarboxylic acid cycle taaffx.123182.1.s1_at carbonic anhydrase family protein −1.35
9 Mitochondrial electron transport ta.1147.3.s1_at NDH-M (SUBUNIT NDH-M OF NAD(P)H:PLASTOQUINONE DEHYDROGENASE COMPLEX) −0.56
10 Cell wall ta.25823.1.a1_at MUM4 | MUM4 (MUCILAGE-MODIFIED 4) −1.12
11 Lipid metabolism ta.4873.1.s1_at FAD8 (FATTY ACID DESATURASE 8) −1.32
12 N metabolism taaffx.12494.1.s1_at nitrogen regulation family protein −0.58
13 Amino acid metabolism ta.23170.1.a1_s_at DHDPS2 (DIHYDRODIPICOLINATE SYNTHASE) −1.06
14 S assimilation ta.19092.1.s1_x_at SIR (sulfite reductase) −0.11
15 Metal handling taaffx.86907.1.s1_at heavy-metal-associated domain-containing protein / copper chaperone (CCH)-related −0.13
16 Secondary metabolism taaffx.12557.1.a1_at terpenoid metabolism −1.08
17 Hormones ta.3526.1.s1_at NFD5 (NUCLEAR FUSION DEFECTIVE 5) −0.99
18 Cofactors and vitamins ta.8003.1.s1_at UbiE/COQ5 methyltransferase family protein −0.70
19 Tetrapyrrole biosynthesis ta.9574.1.s1_at GUN5 (GENOMES UNCOUPLED 5) −1.02
20 Abiotic stress ta.3155.1.s1_at GFA2 (GAMETOPHYTIC FACTOR 2); heat shock protein binding −0.80
20 Stress (biotic and abiotic) ta.27335.1.s1_at ATMLO1/MLO1 (MILDEW RESISTANCE LOCUS O 1) −0.84
21 Redox taaffx.12477.1.s1_at thioredoxin family protein. −1.20
22 Polyamine synthesis ta.24743.2.s1_a_at SPDS1 (SPERMIDINE SYNTHASE 1) −0.15
23 Nucleotide metabolism ta.3179.1.s1_x_at thymidylate kinase family protein −1.20
24 Degradation of xenobiotics ta.7031.3.s1_a_at GLX2-2 (GLYOXALASE 2-2) −0.10
25 C1 metabolism ta.4843.1.a1_at dihydropterin pyrophosphokinase −0.73
26 Misc enzyme families ta.385.2.s1_at ATPRMT11/PRMT11 (ARABIDOPSIS ARGININE METHYLTRANSFERASE 11) −1.20
27 RNA processing ta.26907.1.s1_at ribonuclease T2 family protein −1.20
28 DNA synthesis ta.13372.1.s1_at hydrolase, alpha/beta fold family protein −1.06
29 Protein modification ta.19412.1.s1_at ribosomal protein L20 family protein −1.13
30 Signalling ta.7378.4.s1_at ATARCA (Arabidopsis thaliana Homolog of the Tobacco ArcA); nucleotide binding −1.19
31 Cell cycle, division, vesicle transport and organisation ta.14519.1.s1_x_at regulators of chromosome condensation (RCC1family). −0.89
33 Development ta.7378.4.s1_at ATARCA (Arabidopsis thaliana Homolog of the Tobacco ArcA); nucleotide binding −1.19
34 Transport taaffx.8804.2.s1_at PIP2;5/PIP2D (plasma membrane intrinsic protein 2;5); water channel −1.21