Table 1. Predicted miR-27a Targets among Down-regulated Proteins in LX2/miR-27a Identified by cICAT.

Gene symbol	Accession	Predicted consequentialpairing of target region(top) and miRNA (bottom)		Seed match	Context score	Context scorepercentile	PCT *	H/L
ACLY	NM_001096	Position 697–703 of ACLY 3' UTR	5′ …UGGAAAUGCAGAAAGCUGUGAAA…	7mer-1A	−0.13	73	0.67	0.6597
			||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
AP3D1	NM_001077523	Position 187–193 of AP3D1 3′ UTR	5′ …UGACCAUCCUUUUUUACUGUGAC…	7mer-m8	−0.20	87	<0.1	0.5462
			||| |||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
ATP2A2	NM_170665	Position 2249–2256 of ATP2A2 3′ UTR	5′ …AAAAAAAUCAGCCUUACUGUGAA…	8mer	>−0.03	2	<0.1	0.6095
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
COPA	NM_001098398	Position 1233–1239 of COPA 3′ UTR	5′ …UGAGGACCUAAACUGCUGUGAAA…	7mer-1A	−0.11	63	<0.1	0.6641
			||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
DYNLL2	NM_080677	Position 535–541 of DYNLL2 3′ UTR	5′ …AGAAUAUUCCACUGAACUGUGAU…	7mer-m8	−0.12	71	0.34	0.4487
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
FN1	NM_002026	Position 431–437 of FN1 3′ UTR	5′ …AAGCAUGAUCUUGUU-ACUGUGAU…	7mer-m8	−0.22	89	<0.1	0.5669
			||| |||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
		Position 742–748 of FN1 3′ UTR	5′ …CGGGGGAAAUAAUUCCUGUGAAU…	7mer-1A	−0.13	71	<0.1
			||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
GNPNAT1	NM_198066	Position 175–181 of GNPNAT1 3′ UTR	5′ …GGCUGGUGGGACAUGCUGUGAAU…	7mer-1A	−0.12	68	<0.1	0.5175
			||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
		Position 668–674 of GNPNAT1 3′ UTR	5′ …UACCACUUGUCUUUUCUGUGAAU…	7mer-1A	−0.10	60	<0.1
			|||| ||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGU–GACACUU
H6PD	NM_004285	Position 1513–1519 of H6PD 3′ UTR	5′ …GAGCAUAGGUUGGGGACUGUGAU…	7mer-m8	> −0.02	0	<0.1	0.5198
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
		Position 5755–5761 of H6PD 3′ UTR	5′ …UGUGCCGGAGUGGGAACUGUGAU…	7mer-m8	−0.02	27	<0.1
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
HSD17B12	NM_016142	Position 1071–1078 of HSD17B12 3′ UTR	5′ …AAGAAAGAAUUCAAUACUGUGAA…	8mer	−0.33	97	<0.1	0.3966
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
PAK2	NM_002577	Position 2076–2082 of PAK2 3′ UTR	5′ …CAACGAGAUGAGAAGACUGUGAU…	7mer-m8	> −0.02	2	<0.1	0.5688
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
PPM1B	NM_001033557	Position 177–184 of PPM1B 3′ UTR	5′ …AUUAAACUUUAAAUGACUGUGAA…	8mer	−0.40	99	<0.1	0.4537
			||||| |||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGG–UGACACUU
RAB23	NM_016277	Position 982–988 of RAB23 3′ UTR	5′ …GUCAUUCAGGAGGUCCUGUGAAG…	7mer-1A	−0.01	23	<0.1	0.6407
			||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
SEC61A1	NM_013336	Position 197–204 of SEC61A1 3′ UTR	5′ …GCACUGGCAAAAAGAACUGUGAA…	8mer	−0.30	95	<0.1	0.5849
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
SMAD5	NM_001001419	Position 72–78 of SMAD5 3′ UTR	5′ …ACUUUGAGUACAGAUACUGUGAG…	7mer-m8	−0.20	87	0.75	0.6113
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
		Position 2427–2433 of SMAD5 3′ UTR	5′ …UUAUUGGUGUUUUCUACUGUGAG…	7mer-m8	−0.03	31	<0.1
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU
SPTBN1	NM_178313	Position 2130–2136 of SPTBN1 3′ UTR	5′ …UCAUUUGAUCAUAGCACUGUGAU…	7mer-m8	−0.16	81	<0.1	0.6351
			|||||||
		hsa-miR-27a	3′ CGCCUUGAAUCGGUGACACUU

* P_CT, the probability of conserved targeting.