Figure 7.

Tet TKO results in telomere elongation along with high frequency of T-SCE. (A) Increased telomere length in Tet TKO ESCs. Average telomere length was assessed by qPCR with a telomere repeat-specific primer set and normalized to a single-copy control primer set. (B) Increased telomere length in Tet TKO ESCs as measured by WGBS reads. The table summarizes WGBS reads and uniquely mapped reads. (TTAGGG)₃-containing reads were counted as telomere reads in the raw data and normalized to either total sequencing reads or uniquely mapped reads. (C) Distribution histogram of relative telomere length analyzed by telomere Q-FISH and TFL-TELO software. (D,E) Increased T-SCE in Tet TKO cells. T-SCE events were measured by CO-FISH. (D) Representative images are shown. (E) Quantification of chromosomes with T-SCE events are shown in the bar graph. Statistical significance was calculated by student's t-test; (*) P-value < 0.05; (***) P-value < 0.001.