Table 3.
Primers used for quantitative RT-PCR
| Gene ID/Gene | Product | Sequence (5′ – 3′) | Source |
|---|---|---|---|
| rrsA | 16S ribosomal RNA | F: CCCTAAACGATGCGAACTGGATGT R: AGTTTCAGTCTTGCGACCGTACTC |
Subramoni et al., 2012 |
| rpfF, XOO2869 | RpfF | F: GAGCTGCCACACCATCATCG R: GGCGGAGTACAGATTGCCTTCT |
This study |
| rpfG, XOO2871 | Response regulator | F: TTTCATCACGCTCATCTCGTCGT R: TCTCGAACGCATGTCTCATGTGG |
This study |
| colS, XOO1207 | Two-component system sensor protein | F: TACAAGCGCAAACAGAATCG R: TTGTTACGGGGTCCGAATTA |
This study |
| colR, XOO1208 | Two-component system regulatory protein | F: AGCTTGTTGTCCAGCGAGTC R: ATCGTGCTCGATCTCAACCT |
This study |
| colS, XOO3534 | Two-component system sensor protein, RaxH | F: GATAGCGAGATGCGGATGAT R: ATCTGCAACTGGTCCTGGAG |
This study |
| colR, XOO3535 | Two-component system regulatory protein, RaxR | F: AAGGATCAGGGCGTCGTAGT R: GCTGCTGGTCATTGAAGACA |
This study |
| colS, XOO3762 | Two-component system sensor protein | F: ACGAACGACACCAGATACCC R: CTCACGGTAGCGTTGCCTAT |
This study |
| colR, XOO3763 | Two-component system regulatory protein | F: ACGGCTTGGTCAGGTAGTCATC R: CAAGTCCACGCCGGTGTTGAT |
This study |