Table 4.
Effect of Xanthomonas oryzae pv. oryzae KACC10859 rpfF and rpfG mutations on expression of expression of colS/colR, rpfF and rpfG
| strain | Fold expression change ± standard deviation* | |||||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| XOO1207 | XOO1208 | XOO3534 | XOO3535 | XOO3762 | XOO3763 | rpfF | rpfG | |
| CBNUXO05 (rpfF::EZ-Tn5) | 2.11±0.14a** | 1.90±0.13a | 7.53±0.87a | 2.95±0.11a | 13.31±0.55a | 3.45±0.20a | 0.09±0.01b | 1.44±0.15b |
| CBNUXO05C (rpfF::EZ-Tn5/pVSP61-mcs-sp::rpfF) | 1.23±0.04b | 1.06±0.09b | 1.25±0.15c | 1.12±0.15c | 1.58±0.15bc | 1.32±0.23b | 1.06±0.13a | 0.93±0.12c |
| CBNUXO06 (rpfG::EZ-Tn5) | 1.04±0.05b | 1.12±0.13b | 4.24±0.04b | 1.78±0.12b | 2.13±0.13b | 1.34±0.09b | 0.92±0.06a | 0.01±0d |
| CBNUXO06C (rpfG::EZ-Tn5/pVSP61-mcs-sp::rpfG) | 1.02±0.08b | 1.15±0.02b | 1.33±0.04c | 1.09±0.04c | 1.21±0.04c | 1.03±0.01b | 0.88±0.05a | 4.88±0.19a |
*
The fold expression change (mutant or complemented mutant/wild type) was calculated using 2−ΔΔCt with three replicates.
**
Means with the same letter are not significantly different by Turkey’s HSD test using SAS 9.2.