Fig. 3.

Geminivirus susceptibility of AtREM4s. (A) The gene structures and T-DNA insertion sites. Black boxes are exons, and gray boxes are untranslated regions. Intergenic regions or introns are marked with lines. Expression levels of AtREM4s in the double mutants as assayed by RT-PCR. (B) For the geminivirus infection experiment, Agrobacterium stains containing BCTV, BSCTV and control vector, pMON, were inoculated in the crown of the rosette of four-week-old WT and double mutant plants using a needle. (C) BSCTV infection experiments were performed using WT, AtREM4.1-1, AtREM4.2-1, AtREM4.1-1/4.2-1, 35S::AtREM4.1-CFP and 35S::AtREM4.2-CPF. (D) For severely infected plants, plant symptom severity rates were classified as previously described (Park et al., 2011). (E) For RT-PCR, total RNA was isolated from the plants that were infected with BSCTV, and RT-PCR was performed. The Actin-2 gene was used as a loading control for PCR.