Figure 2.

Role of Sox4 in ALL in vivo. (A) and (B) Effect of Sox4 depletion in vitro on the development of leukemia in a NOD/SCID transplantation model. Each mouse was injected via the tail vein with luciferase-labeled and p190 BCR-ABL-transformed Sox4^fl/flSE-Cre or Sox4^fl/+SE-Cre pro-B cells (10⁶ cells/mouse) and development of leukemia was monitored weekly by bio-imaging. Overall survival was analyzed by the Kaplan-Meier method. (C) Analysis of Sox4 mRNA expression by real-time RT-PCR in ALL cells from bone marrow of mice transplanted with BCR-ABL⁺ Sox4^fl/+Cre or Sox4^fl/flCre cells. (D) and (E) Effect of Sox4 depletion in vivo on the progression of leukemia in a NOD/SCID transplantation model. Sox4^fl/fl;Cre-ER;eYFP pro-B cells that were transformed with BCR-ABL and labeled with luciferase were transplanted into NOD/SCID mice (3×10⁶ cells/mouse) and the deletion of floxed Sox4 was induced with tamoxifen (TAM) after the onset of leukemia. Leukemia progression was monitored weekly by bio-imaging. (F) Analysis of Sox4 mRNA expression by real-time RT-PCR in ALL cells from bone marrow of mice transplanted with Sox4^fl/fl;Cre-ER;eYFP pro-B cells and treated with TAM or vehicle. The relative mRNA levels were normalized to the level of Gapdh mRNA. Values are means ± SD (n=3). *P<0.05, **P<0.01, ***P<0.001