Figure 4.

Effect of Tcf7l1 in ALL. (A) Decreased Tcf7l1 mRNA expression in Tcf7l1 shRNA cells compared with NT shRNA cells. The relative mRNA levels of Tcf7l1 gene were normalized to the level of Gapdh mRNA. (B) and (C) Effect of Tcf7l1 mRNA knockdown on the proliferation and colony formation of BCR-ABL–transformed wild-type pro-B cells. The mean colony numbers were 10±2.12 and 59±2.83 for Tcf7l1 knockdown and control cells, respectively. Tcf7l1 shRNA: Tcf7l1 specific shRNA; NT shRNA: non-targeting shRNA. (D) Analysis of Tcf7l1 mRNA expression in transformed Sox4^fl/flSE-Cre cells transfected with Tcf7l1 overexpression vector or control vector. (E) and (F) Effect of Tcf7l1 overexpression on cell proliferation and colony formation of transformed Sox4^fl/flSE-Cre pro-B cells. The mean colony numbers were 7±2.51 and 22±2.0 for control cells and cells with Tcf7l1 overexpression, respectively. Data are representative of three independent experiments. Values are means ± SD (n=3). *P< 0.05, **P<0.01, ***P<0.001