Table 2. Distribution of the predominant inner and outer membrane phosphatidylethanolamines and phosphatidylglycerols in planktonic and biofilms Pseudomonas aeruginosa (results are given with 95% confidence interval).
| PEs | PGs | |||||||||||
| PL* | R | R’ | mass | plankt | BF 1 day | BF 2 days | BF 6 days | mass | plankt | BF 1 day | BF 2 days | BF 6 days |
| 32∶1 | C16∶0 | C16∶1 | 688.49 | 4.8±0.6 | 6.6±0.5 | 6.6±0.3 | 6.0±0.5 | 719.49 | 4.4±2.0 | 4.7±0.8 | 4.8±0.9 | 4.9±1.1 |
| C14∶0 | C18∶1 | 5.4±0.6 | 5.6±0.5 | 7.3±0.5 | 7.3±0.5 | n.d. | n.d. | n.d. | n.d. | |||
| CH3-C14∶0[traces] | C17∶0cyc [traces] | |||||||||||
| 32∶0 | C16∶0 | C16∶0 | 690.51 | 8.6±0.6 | 8.2±0.5 | 11.0±0.3 | 8.8±0.5 | 721.50 | 5.6±2.0 | 7.2±0.8 | 4.4±0.9 | 4.4±1.1. |
| C14∶0 | C18∶0 | 7.0±0.5 | 5.4±0.5 | 13.6±0.5 | 5.6±0.5 | n.d. | n.d. | n.d. | n.d. | |||
| 33∶1 | C16∶0 | C17∶0cyc(9,10) | 702.51 | 5.9±0.4 | 3.5±0.3 | 4.6±0.3 | 14.5±0.7 | 733.50 | 6.2±0.8 | 6.0±0.5 | 7.6±0.5 | 12.9±0.5 |
| CH3-C16∶0 | C16∶1 | 5.9±0.5 | 4.6±0.5 | 2.6±0.3 | 21.1±0.9 | 9.5±0.8 | 5.9±0.5 | 5.7±0.5 | 2.1±0.5 | |||
| 33∶0 | C16∶0 | CH3-C16∶0 | 704.52 | 735.52 | 2.7±0.8 | 4.2±0.5 | 2.5±0.5 | 4.1±0.5 | ||||
| CH3-C14∶0 | C18∶0 | 2.6±0.8 | 2.5±0.7 | 2.1±0.5 | 2.6±0.6 | 8.2±0.5 | 2.2±0.7 | 3.0±0.5 | 3.1±0.6 | |||
| 34∶2 | C16∶1 | C18∶1 | 714.51 | 5.2±1.6 | 13.7±0.7 | 7.7±0.9 | 8.2±1.0 | 745.50 | 2.4±0.5 | 7.4±0.5 | 3.5±0.5 | 5.3±0.5 |
| 6.2±0.8 | 11.7±0.9 | 8.2±0.9 | 6.5±0.9 | 4.1±0.8 | 7.1±0.6 | 8.0±0.6 | 10.0±0.6 | |||||
| 34∶1 | C16∶0 | C18∶1 | 716.52 | 44.8±1.8 | 35.3±0.7 | 35.4±0.9 | 30.7±1.0 | 747.52 | 21.9±0.5 | 17.1±0.5 | 17.9±0.5 | 19.5±0.5 |
| C16∶1 | C18∶0 | 45.8±1.0 | 31.4±0.9 | 45.2±0.9 | 26.9±0.8 | 25.9±0.8 | 23.4±0.7 | 33.4±0.7 | 28.2±0.8 | |||
| CH3-C14∶0 [traces] | C19∶0cyc [traces] | |||||||||||
| 35∶2 | C16∶1 | C19∶0cyc(11, 12) | 728.52 | 4.2±0.8 | 4.0±0.5 | 4.6±0.5 | 4.6±0.5 | 759.59 | n.d. | 6.9±0.5 | 4.9±0.5 | n.d. |
| C17∶0cyc(9,10) | C18∶1 | 4.0±1.0 | 5.9±0.5 | 2.6±0.5 | 4.3±0.5 | 3.6±0.5 | 8.8±0.5 | 4.3±0.5 | 6.7±0.5 | |||
| 35∶1 | C16∶0 | C19∶0cyc(11, 12) | 730.54 | 17.6±0.8 | 9.1±0.5 | 13.1±0.5 | 12.0±0.5 | 761.53 | 31.6±0.8 | 13.7±0.5 | 25.7±0.5 | 27.3±0.5 |
| CH3-C16∶0 | C18∶1 | 15.0±1.0 | 12.8±0.8 | 5.2±0.5 | 11.8±0.9 | 23.5±0.8 | 20.4±0.8 | 13.9±0.5 | 22.9±0.9 | |||
| 36∶2 | C18∶1 | C18∶1 | 742.54 | 2.8±0.6 | 4.4±0.5 | 3.2±0.5 | 3.6±0.5 | 773.53 | 5.0±0.5 | 5.3±0.5 | 6.2±0.5 | 6.5±0.5 |
| C17∶0cyc(9,10) | C19∶0cyc(11, 12) | 2.7±0.6 | 5.1±0.5 | 2.3±0.5 | 3.4±0.5 | 5.0±0.5 | 10.4±0.5 | 5.2±0.5 | 7.6±0.5 | |||
| Sum | 93.9 | 84.8 | 86.2 | 88.5 | 74.8 | 67.2 | 71.3 | 78.6 | ||||
| 91.9 | 85.0 | 89.2 | 89.5 | 79.9 | 78.2 | 80.2 | 80.5 | |||||
Values in straight correspond to the internal membrane - Values in italic correspond to the outer membrane.
*Total number of C: Number of equivalent unsaturation – R and R' represent the fatty acid moiety with no indication of their position.
Note that a cyclopropylation is equivalent to an unsaturation in terms of molecular mass (e.g. the molecular masses of .C17∶0cyc and C17∶1 are identical).