Fig. 6.

Bcl-2 protein family expression in SH-SY5Y cells subjected to oxidative stress. mRNA levels for Bcl-2 (a), Hrk (b) and Bax (c) were evaluated using real-time PCR after incubation with C2-ceramide (25 μM, 3 h). The value expresses the fold of gene stimulation normalised against Actb (β-actin). Data represent the mean value ± SEM for three separate experiments with three replications. **p < 0.01, *p < 0.05 versus control SH-SY5Y cells, ^## p < 0.01, ^# p < 0.05 versus C2-ceramide treated SH-SY5Y cells by using one-way ANOVA followed by the Newman-Keuls post hoc test