Table 1. Values of mitochondrial respiratory chain complexes in cultures of normal chondrocytes treated with 20 ng/ml T-2 toxin for 5 days.
Control | 20 ng/ml T-2 | 20 ng/ml T-2+1 µM Se | |
n | 8 | 8 | 8 |
Age (years old) | 45.38±8.78 | 45.38±8.78 | 45.38±8.78 |
Proteins (mg/ml) | 2.98±0.47 | 2.55±0.68 | 2.75±0.55 |
CS enzymatic activity (nmol/minute/mg protein) | 95.39±15.62 | 88.99±12.21 | 94.21±13.70 |
Mitochondrial complex activity† | |||
Complex I | 33.54±6.84 | 28.71±5.30 | 30.94±6.84 |
Complex II | 13.65±2.58 | 11.24±1.94 | 12.64±2.53 |
Complex III | 55.35±7.29 | 47.70±3.88* | 53.32±5.24 |
Complex IV | 50.41±5.79 | 39.91±5.36* | 46.65±6.50# |
Complex V | 53.44±9.84 | 42.26±8.46* | 51.95±8.83# |
Values are the mean ± SD. CS = citrate synthase. †CS-corrected complex activity is expressed as (nmoles/minute/mg protein)/(CS specific activity) ×100. *P<0.05 versus normal chondrocytes. #P<0.05 versus 20 ng/ml T-2 toxin group. Complex I = rotenone-sensitive NADH-coenzyme Q1 reductase; complex II = succinate dehydrogenase; complex III = antimycin-sensitive ubiquinol cytochrome c reductase; complex IV = cytochrome c oxidase; complex V = ATP synthase.