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. 2014 Sep 29;9(9):e108394. doi: 10.1371/journal.pone.0108394

Table 1. Values of mitochondrial respiratory chain complexes in cultures of normal chondrocytes treated with 20 ng/ml T-2 toxin for 5 days.

Control 20 ng/ml T-2 20 ng/ml T-2+1 µM Se
n 8 8 8
Age (years old) 45.38±8.78 45.38±8.78 45.38±8.78
Proteins (mg/ml) 2.98±0.47 2.55±0.68 2.75±0.55
CS enzymatic activity (nmol/minute/mg protein) 95.39±15.62 88.99±12.21 94.21±13.70
Mitochondrial complex activity†
Complex I 33.54±6.84 28.71±5.30 30.94±6.84
Complex II 13.65±2.58 11.24±1.94 12.64±2.53
Complex III 55.35±7.29 47.70±3.88* 53.32±5.24
Complex IV 50.41±5.79 39.91±5.36* 46.65±6.50#
Complex V 53.44±9.84 42.26±8.46* 51.95±8.83#

Values are the mean ± SD. CS = citrate synthase. †CS-corrected complex activity is expressed as (nmoles/minute/mg protein)/(CS specific activity) ×100. *P<0.05 versus normal chondrocytes. #P<0.05 versus 20 ng/ml T-2 toxin group. Complex I = rotenone-sensitive NADH-coenzyme Q1 reductase; complex II = succinate dehydrogenase; complex III = antimycin-sensitive ubiquinol cytochrome c reductase; complex IV = cytochrome c oxidase; complex V = ATP synthase.