. Author manuscript; available in PMC: 2014 Oct 2.

Published in final edited form as: Biopharm Drug Dispos. 2007 Sep;28(6):263–273. doi: 10.1002/bdd.551

Table 1.

Reaction conditions for quantitative real-time reverse transcriptase-polymerase chain reaction (QRT RT-PCR)

Oligonucleotide	Concentration	Sequence 5′-3′
Forward primer	300nM	GACATGGTGTTTTCAGATTGCAA
Reverse primer	300nM	CAGGACAGGATGGGAACATTG
FAM-labeled probe	100nM	TACGAACCACTGGCCAACCTCAGCA
MgCl₂	3mM	—
DNTP	1mM	—