Panneuroral marker (ICC) |
1:3000 |
Millipore |
MAB2300 |
Biotinylated Donkey anti mouse |
1:500 |
Jackson Immuno Research |
715-065-150 |
Glial Fibrillary Acidic protein (GFAP) (WB) |
1:20,000 |
Sigma |
G9269 |
HRP-conj. Goat anti Rabbit |
1:7,500 |
Thermo Scientific |
31460 |
Glial Fibrillary Acidic protein (GFAP) (ICC) |
1:10,000 |
Sigma |
G9269 |
Cy3-conj. Donkey anti Rabbit |
1:500 |
Jackson Immuno Research |
711-165-152 |
Nestin (ICC) |
1:1000 |
Sigma |
N5413 |
Cy3-conj. Donkey anti Rabbit |
1:500 |
Jackson Immuno Research |
711-165-152 |
Neuron-specific Enolase (NSE) (WB) |
1:3000 |
Abcam |
ab16873 |
HRP-conj. Goat anti Rabbit |
1:7,500 |
Thermo Scientific |
31460 |
Synaptosomal-associated Protein of 25 kDa (SNAP25) (WB) |
1:5000 |
Millipore |
MAB331 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |
Post Synaptic Density Protein of 95 kDa (PSD-95) (WB) |
1:3000 |
NeuroMab |
75-028 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |
β actin (WB) |
1:100,000 |
Sigma |
A5441 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |
5-Hydroxytryptamine (5-HT) (ICC) |
1:1000 |
ImmunoStar |
20080 |
Cy3-conj. Donkey anti Rabbit |
1:500 |
Jackson Immuno Research |
711-165-152 |
Glulamate Decarboxylase (GAD65/67) (WB) |
1:1000 |
Millipore |
AB1511 |
HRP-conj. Goat anti Rabbit |
1:7,500 |
Thermo Scientific |
31460 |
Tyrosine Hyroxylase (WB) |
1:2000 |
Millipore |
MAB318 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |
Amyloid Precursor Protein (APP) (22C11 clone) |
1:3000 |
Millipore |
MAB348 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |
α-tubulin |
1:100,000 |
Sigma |
T9026 |
HRP-conj. Goat anti Mouse |
1:3000 |
Rockland |
610-1319 |