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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Isr J Chem. 2013 Jun;53(6-7):469–483. doi: 10.1002/ijch.201300009

Table 3.

Base pair stability ranking from relative thermal denaturation at pH 7.9.[a]

2 H-bond base pair Melting temp. (°C) 3 H-bond base pair Melting temp. (°C)
graphic file with name nihms574158t1.jpg Reported higher than U-A[b] graphic file with name nihms574158t2.jpg 61.5, 63.3
graphic file with name nihms574158t3.jpg 52.9, 55.9 graphic file with name nihms574158t4.jpg Reported similar to a G:C pair[c]
graphic file with name nihms574158t5.jpg 54.4 graphic file with name nihms574158t6.jpg 58.5, 59.5
graphic file with name nihms574158t7.jpg 52.1, 54.7 graphic file with name nihms574158t8.jpg 56.9, 58.4
graphic file with name nihms574158t9.jpg 52.8, 54.6 graphic file with name nihms574158t10.jpg 56.7
graphic file with name nihms574158t11.jpg 47.5 graphic file with name nihms574158t12.jpg 47.5
[a]

Data from reference [59].

[b]

Data from reference [60]. Data from this study compared 7mer oligos of RNA duplexes monitoring one substitution. The s2U :A base pair substantially increased the melting temperature to 57.4° in comparison to 51.6° for a U :A pair.

[c]

According to references [51,62].