Quantitative analysis of MSC adhesion to H-terminated USND, Ti-6AI-4V and CoCrMo. Prior to cell seeding, the surfaces were either pre-coated with serum (FBS) or left uncoated. Cells were cultured for 90 minutes, followed by mechanical agitation to remove loosely bound MSCs. Remaining attached cells were quantified by assay for DNA content. Adhesion of MSCs to USND was found to be intermediate between that of Ti-6AI-4V and CoCrMo, in both serum-free conditions and in the presence of serum. * denotes p < 0.05. Reprinted with permission from Clem et al., Biomaterials 29 (2008) 3461–3468 © Elsevier 2008.