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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: J Cancer Ther. 2013 Jun 1;4(4):898–906. doi: 10.4236/jct.2013.44101

Figure 5.

Figure 5

Effect of Nef peptides on caspase-3 activation in MDA-MB231 cells. Caspase-3 activation was determined by cleavage of the 32 kDa pro-caspase-3 protein into two smaller 17 kDa and 12 kDa caspase-3 proteins. Nef protein (lane 4) and Nef peptides [Nef 41 – 60 (lane 5), Nef-M1 (lane 6)] were efficient in inducing apoptosis, as determined after exposure for 48 hr, as determined by WBs, which show the activated caspase-3 in comparison to untreated (lane 1) cells and cells treated with Nef-sM1 (lane 2) or Nef 171 – 180 (lane 3).