Skip to main content
. 2014 Aug 13;3(10):1125–1137. doi: 10.5966/sctm.2013-0128

Figure 6.

Figure 6.

Col2.3 GFPemd cells are embedded in human bone sialoprotein (BSP) staining matrix. An adjacent section to those used in Figures 4 and 5 was scanned with dark-field optics to show the bone matrix ([A3], white). Human BSP immunostaining ([A1] and [A2], red) was applied to the section after decalcification. Col2.3GFPemd-positive cells were identified by green fluorescence ([A1] and [A2], green). Cell nuclei were shown by 4′,6-diamidino-2-phenylindole ([A1], blue). Scale bar = 200 μm. (B): Higher magnification of the boxed region in (A1) was imaged. The white star marks human BSP negative mouse bone. White arrowheads mark the hydroxyapatites within the remaining scaffold. Scale bar = 50 μm. (C): Higher magnification of boxed region in (B) was imaged with (C2) or without (C1) green fluorescence. The white arrows in (C1) and (C2) point to Col2.3GFPemd-positive cells (C2) embedded in human BSP staining (C1). Scale bar = 20 μm.