Figure 7.

Col2.3GFPemd-positive cells were more enriched in defects implanted with C341-6-derived mesenchymal cells. (A): Col2.3GFPemd-positive human osteoblasts (green) were detected in calvarial defect implanted with C341-6 derived mesenchymal cells. Samples were processed as described in Figure 4. White: dark-field. Red: AC labeling. Blue: 4′,6-diamidino-2-phenylindole nuclei staining. Yellow: AP staining. Arrows in (A) and (B): AP staining of human osteoblasts. Arrowhead in (A) and (B): AP staining of mouse osteoblasts. Boxed region in (A) is shown in (B). Color assignments for (B1) and (B2) are the same as for Figure 4B1 and 4B3, respectively. Scale bars = 200 μm (A) and 50 μm (B).