Figure 5.

Rapamycin-enhanced gene marking in differentiated lineage progenies. Phenotypic analysis of GFP+ cells in CD33+, CD14+, and CD15+ myeloid cells, as well as glycophorin A+ erythroid population. CD34+ cells were prestimulated overnight in SFT6 and transduced with LV3 (multiplicity of infection, 10) in the absence (top) and presence (bottom) of rapamycin during transduction. Transduced cells were harvested after overnight transduction and plated in myeloid differentiation bulk culture as described in Materials and Methods. The plots shown result from day 14 of culture. Abbreviation: GFP, green fluorescent protein; LV, lentivirus; SFT6, stem cell factor, flt-3 ligand, thrombopoietin, and interleukin-6.