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. 2014 Sep 9;3(10):1169–1181. doi: 10.5966/sctm.2014-0097

Figure 5.

Figure 5.

De novo generation of Tregs by pericytes is mediated through PD-L1/2 and TGF-β. (A): Expression of PD-L1 by CD45− brain pericytes and CD45+ T cells in cocultures. Left panel: CD45-labeled, IgG control cells. The dot plot is representative of the CD25− T-cell subset cultured with hPSC or placenta pericytes for n = 4 independent experiments. (B, C): Concentrations of secreted TGF-β in the conditioned medium of cultivated brain and hPSC pericytes (B) or cocultures of pericytes and CD3+CD25− T cells (C) in the indicated treatments. (D): Representative dot plot analyses of the expression of CD127 and FoxP3 by CD4+ T cells as a dependency on the level of expression of CD25 in the presence or absence of TGF-β tyrosine kinase inhibitor (SB431542) or blocking antibodies against PD-L1/2. Abbreviations: hPSC, human pluripotent stem cell; PD-L1/2, programmed cell death ligand 1/2; TGF, transforming growth factor.