Table 1.
PTMs of T. gondii, yeast and human Hsp90 members
| TgHsp90 | YHsp90 | HuHsp90α | HuHsp90β | Domain | |
|---|---|---|---|---|---|
| Phosphorylation | – | – | T5 | – | N domain |
| – | – | T7 | – | ||
| T23 | T22 | T36 | T31 | ||
| Y25 | Y24 | Y38 | Y33 | ||
| Y48 | Y47 | Y61 | Y56 | ||
| – | S49 | S63 | S58 | ||
| T52 | S51 | T65 | T60 | ||
| – | – | S68 | S63 | ||
| – | T58 | T72 | T67 | ||
| T75 | – | T88 | T83 | ||
| T77 | – | T90 | T85 | ||
| Y186 | Y184 | Y197 | Y192 | ||
| S200 | S198 | S211 | S206 | ||
| T220 | – | S231 | S226 | Linker | |
| S222 | – | – | – | ||
| – | – | S252 | – | ||
| – | – | S263 | S255 | ||
| – | S282 | – | – | Middle domain | |
| T282 | T285 | T305 | T297 | ||
| Y286 | Y289 | Y309 | Y300 | ||
| S292 | S295 | S315 | S307 | ||
| T294 | S297 | T317 | T309 | ||
| T331 | S334 | – | – | ||
| S376 | S379 | S399 | S391 | ||
| T430 | T433 | S453 | S445 | ||
| – | – | S460 | S452 | ||
| S445 | S450 | S470 | S462 | ||
| S453 | S456 | S476 | S468 | ||
| S464 | – | – | – | ||
| Y469 | Y472 | Y492 | Y484 | ||
| Y470 | Y473 | Y493 | Y485 | ||
| S482 | S485 | S505 | S497 | ||
| – | – | – | S532 | ||
| Y581 | – | Y604 | Y596 | C domain | |
| S600 | S602 | S623 | |||
| Y604 | Y606 | Y627 | Y619 | ||
| – | S616 | – | – | ||
| – | S619 | – | – | ||
| S654 | S657 | S677 | S668 | ||
| – | S663 | T683 | T675 | ||
| – | – | T725 | – | ||
| – | – | S726 | S718 | ||
| Acetylationa | K271 | K274 | K294 | K286 | |
| K384 | K387 | K407 | K399 | ||
| K559 | – | K582 | K574 | ||
| S-nitrosylation | C549 (ND) | – | C572 | C564 | |
| C574 (ND) | – | C597 | C589 |
a
Data only include acetylated lysines identified in T. gondii and human Hsp90. Other lysines that were detected in human Hsp90 are not included.
ND: indicates not determined; Normal font: PTMs identified; italic font: orthologue residue; –: non phosphorylated (Y,T, S), acetylated (K) or S-nitrosylated (C) residue; Obs.: Hsp90 domains are only identified for phosphorylation section.