| Group | Assay condition | Notes |
| A | CCL-23 cells alone | |
| B | CCL-23 cells + pneumococci | |
| C | CCL-23 cells + pneumococci + heparin | Heparin blocks pneumococcal adherence to cell surface glycosylaminoglycans16 and is used as a control |
| D | CCL-23 cells + pneumococci + S. salivarius [high] | Approximately 1 pneumococcus: 10 S. salivarius |
| E | CCL-23 cells + pneumococci + S. salivarius [med] | Approximately 1 pneumococcus: 1 S. salivarius |
| F | CCL-23 cells + pneumococci + S. salivarius [low] | Approximately 10 pneumococcus: 1 S. salivarius |
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